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KMID : 1094020230400050323
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Journal of Veterinary Clinics
2023 Volume.40 No. 5 p.323 ~ p.329
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Isolation of Peripheral Blood-Derived Mesenchymal Stem Cells in Mares and Foals
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Oh Ye-Eun
Lee Eun-Bee
Seo Jong-Pil
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Abstract
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Peripheral blood-derived mesenchymal stem cells (PB-MSCs) have shown promise in cell-based therapy, as they can be harvested with ease through minimally invasive procedures. This study aimed to isolate PB-MSCs from foals and mares and to compare the proliferation and cellular characteristics of the PB-MSCs between the two groups. Six pairs of mares and their foals were used in this study. MSCs were isolated from PB by direct plating in a tissue culture medium, and cell proliferation (population doubling time [PDT], and colony-forming unit-fibroblast assay [CFU-F]), and characterization (morphology, plastic adhesiveness, colony formation, trilineage differentiation) were examined. There was no significant difference in the PB-MSC yield, CFU-F, and PDT between the mares and foals. PB-MSCs from both mares and foals showed typical MSC characteristics in terms of spindle-shaped morphology, plastic adhesive properties, formation of colonies, trilineage differentiation. These results suggest that PB-MSCs isolated from horses, both adult horses, and foals, can be used for equine cell-based therapy.
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KEYWORD
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peripheral blood-derived mesenchymal stem cells, MSCs isolation, horse
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